Mapping protein–ligand interactions by footprinting, a radical idea
نویسندگان
چکیده
منابع مشابه
Mapping of mitochondrial RNA-protein interactions by digital RNase footprinting.
Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific...
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Measurements from hydroxyl radical footprinting (HRF) provide rich information about the solvent accessibility of amino acid side chains of a protein. Traditional HRF data analyses focus on comparing the difference in the modification/footprinting rate of a specific site to infer structural changes across two protein states, e.g., between a free and ligand-bound state. However, the rate informa...
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We used a high flux synchrotron X-ray beam to map the structure of 16S rRNA and RNase P in viable bacteria in situ. A 300 ms exposure to the X-ray beam was sufficient for optimal cleavage of the phosphodiester backbone. The in vivo footprints of the 16S rRNA in frozen cells were similar to those obtained in vitro and were consistent with the predicted accessibility of the RNA backbone to hydrox...
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Rolling adhesion, in which cells passively roll along surfaces under shear flow, is a critical process involved in inflammatory responses and cancer metastasis. Surface adhesion properties regulated by adhesion receptors and membrane tethers are critical in understanding cell rolling behavior. Locally, adhesion molecules are distributed at the tips of membrane tethers. However, how functional a...
متن کاملOn a new type of stability of a radical cubic functional equation related to Jensen mapping
The aim of this paper is to introduce and solve the radical cubic functional equation $fleft(sqrt[3]{x^{3}+y^{3}}right)+fleft(sqrt[3]{x^{3}-y^{3}}right)=2f(x)$. We also investigate some stability and hyperstability results for the considered equation in 2-Banach spaces.
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ژورنال
عنوان ژورنال: Structure
سال: 1998
ISSN: 0969-2126
DOI: 10.1016/s0969-2126(98)00071-9